Proteomic profile of bone “collagen” extracted for stable isotopes: Implications for bulk and single amino acid analyses

Rationale Protein studies in archaeology and paleontology have been dominated by stable isotope to understand diet trophic levels, but recent applications of proteomic techniques resulted a more complete understanding protein diagenesis than isotopes alone. In analyses, samples are retained or discarded based on their properties. Proteomics can directly determine what proteins present within the sample may be able allow previously analyzed. Methods that had analyzed for isotopes, including those with marginal poor quality, were characterized liquid chromatography/mass spectrometry using an LTQ Orbitrap Velos mass spectrometer after separation Dionex Ultimate 3000 LC system. Data MetaMorpheus custom R scripts. Results We found variety addition collagen, although collagen I was majority (most >80%). also positive correlation between total deamidation wt% N, suggesting impact overall nitrogen signal bulk analyses. The amino acid profiles samples, reflect expected percentages, allowing use single Conclusions All regardless quality high concentrations I, making interpretations dietary routing reasonably valid. profile these recovered